A nanobeads amplified QCM immunosensor for the detection of avian influenza virus H5N1
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Auteurs : DUJUAN LI [République populaire de Chine, États-Unis] ; JIANPING WANG [République populaire de Chine] ; RONGHUI WANG [États-Unis] ; YANBIN LI [États-Unis] ; Daad Abi-Ghanem [États-Unis] ; Luc Berghman [États-Unis] ; Billy Hargis [États-Unis] ; HUAGUANG LU [États-Unis]Source :
- Biosensors & bioelectronics [ 0956-5663 ] ; 2011.
Descripteurs français
- Pascal (Inist)
English descriptors
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Abstract
As a potential pandemic threat to human health, there has been an urgent need for rapid detection of the highly pathogenic avian influenza (AI) H5N1 virus. In this study, magnetic nanobeads amplification based quartz crystal microbalance (QCM) immunosensor was developed as a new method and application for AI H5N1 virus detection. Polyclonal antibodies against AI H5N1 virus surface antigen HA (Hemagglutinin) were immobilized on the gold surface of the QCM crystal through self-assembled monolayer (SAM) of 16-mercaptohexadecanoic acid (MHDA). Target H5N1 viruses were then captured by the immobilized antibodies, resulting in a change in the frequency. Magnetic nanobeads (diameter, 30 nm) coated with anti-H5 antibodies were used for further amplification of the binding reaction between antibody and antigen (virus). Both bindings of target H5N1 viruses and magnetic nanobeads onto the crystal surface were further confirmed by environmental scanning electron microscopy (ESEM). The QCM immunosensor could detect the H5N1 virus at a titer higher than 0.0128 HA unit within 2 h. The nanobeads amplification resulted in much better detection signal for target virus with lower titers. The response of the antibody-antigen (virus) interaction was shown to be virus titer-dependent, and a linear correlation between the logarithmic number of H5N1 virus titers and frequency shift was found from 0.128 to 12.8 HA unit. No significant interference was observed from non-target subtypes such as AI subtypes H3N2, H2N2, and H4N8. The immunosensor was evaluated using chicken tracheal swab samples. This research demonstrated that the magnetic nanobeads amplification based QCM immunosensor has a great potential to be an alternative method for rapid, sensitive, and specific detection of AI virus H5N1 in agricultural, food, environmental and clinical samples.
Affiliations:
- République populaire de Chine, États-Unis
- Arkansas, Pennsylvanie, Texas, Zhejiang
- Hangzhou
- Université de Zhejiang
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sortKey="Dujuan Li" sort="Dujuan Li" uniqKey="Dujuan Li" last="Dujuan Li">DUJUAN LI</name><affiliation wicri:level="4"><inist:fA14 i1="01"><s1>College of Biosystems Engineering and Food Science, Zhejiang University, 866 Yuhangtang Road</s1><s2>Hangzhou 310058</s2><s3>CHN</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ></inist:fA14><country>République populaire de Chine</country><placeName><settlement type="city">Hangzhou</settlement><region type="province">Zhejiang</region></placeName><orgName type="university">Université de Zhejiang</orgName><placeName><settlement type="city">Hangzhou</settlement><region type="province">Zhejiang</region></placeName></affiliation><affiliation wicri:level="2"><inist:fA14 i1="02"><s1>Department of Biological and Agricultural Engineering, University of Arkansas</s1><s2>Fayetteville, AR 72701</s2><s3>USA</s3><sZ>1 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ></inist:fA14><country>États-Unis</country><placeName><region type="state">Arkansas</region></placeName></affiliation></author><author><name sortKey="Jianping Wang" sort="Jianping Wang" uniqKey="Jianping Wang" last="Jianping Wang">JIANPING WANG</name><affiliation wicri:level="4"><inist:fA14 i1="01"><s1>College of Biosystems Engineering and Food Science, Zhejiang University, 866 Yuhangtang Road</s1><s2>Hangzhou 310058</s2><s3>CHN</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ></inist:fA14><country>République populaire de Chine</country><placeName><settlement type="city">Hangzhou</settlement><region type="province">Zhejiang</region></placeName><orgName type="university">Université de Zhejiang</orgName><placeName><settlement type="city">Hangzhou</settlement><region type="province">Zhejiang</region></placeName></affiliation></author><author><name sortKey="Ronghui Wang" sort="Ronghui Wang" uniqKey="Ronghui Wang" last="Ronghui Wang">RONGHUI WANG</name><affiliation wicri:level="2"><inist:fA14 i1="02"><s1>Department of Biological and Agricultural Engineering, University of Arkansas</s1><s2>Fayetteville, AR 72701</s2><s3>USA</s3><sZ>1 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ></inist:fA14><country>États-Unis</country><placeName><region type="state">Arkansas</region></placeName></affiliation></author><author><name sortKey="Yanbin Li" sort="Yanbin Li" uniqKey="Yanbin Li" last="Yanbin Li">YANBIN LI</name><affiliation wicri:level="2"><inist:fA14 i1="02"><s1>Department of Biological and Agricultural Engineering, University of Arkansas</s1><s2>Fayetteville, AR 72701</s2><s3>USA</s3><sZ>1 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ></inist:fA14><country>États-Unis</country><placeName><region type="state">Arkansas</region></placeName></affiliation><affiliation wicri:level="2"><inist:fA14 i1="03"><s1>Center of Excellence for Poultry Science, University of Arkansas</s1><s2>Fayetteville, AR 72701</s2><s3>USA</s3><sZ>4 aut.</sZ><sZ>7 aut.</sZ></inist:fA14><country>États-Unis</country><placeName><region type="state">Arkansas</region></placeName></affiliation></author><author><name sortKey="Abi Ghanem, Daad" sort="Abi Ghanem, Daad" uniqKey="Abi Ghanem D" first="Daad" last="Abi-Ghanem">Daad Abi-Ghanem</name><affiliation wicri:level="2"><inist:fA14 i1="04"><s1>Department of Poultry Science and Veterinary Pathobiology, Texas A&M University</s1><s2>College Station, TX 77843</s2><s3>USA</s3><sZ>5 aut.</sZ><sZ>6 aut.</sZ></inist:fA14><country>États-Unis</country><placeName><region type="state">Texas</region></placeName></affiliation></author><author><name sortKey="Berghman, Luc" sort="Berghman, Luc" uniqKey="Berghman L" first="Luc" last="Berghman">Luc Berghman</name><affiliation wicri:level="2"><inist:fA14 i1="04"><s1>Department of Poultry Science and Veterinary Pathobiology, Texas A&M University</s1><s2>College Station, TX 77843</s2><s3>USA</s3><sZ>5 aut.</sZ><sZ>6 aut.</sZ></inist:fA14><country>États-Unis</country><placeName><region type="state">Texas</region></placeName></affiliation></author><author><name sortKey="Hargis, Billy" sort="Hargis, Billy" uniqKey="Hargis B" first="Billy" last="Hargis">Billy Hargis</name><affiliation wicri:level="2"><inist:fA14 i1="03"><s1>Center of Excellence for Poultry Science, University of Arkansas</s1><s2>Fayetteville, AR 72701</s2><s3>USA</s3><sZ>4 aut.</sZ><sZ>7 aut.</sZ></inist:fA14><country>États-Unis</country><placeName><region type="state">Arkansas</region></placeName></affiliation></author><author><name sortKey="Huaguang Lu" sort="Huaguang Lu" uniqKey="Huaguang Lu" last="Huaguang Lu">HUAGUANG LU</name><affiliation wicri:level="2"><inist:fA14 i1="05"><s1>Animal Diagnostic Laboratory, Penn State University</s1><s2>State College, PA 16802</s2><s3>USA</s3><sZ>8 aut.</sZ></inist:fA14><country>États-Unis</country><placeName><region type="state">Pennsylvanie</region></placeName></affiliation></author></analytic><series><title level="j" type="main">Biosensors & bioelectronics</title><title level="j" type="abbreviated">Biosens. bioelectron.</title><idno type="ISSN">0956-5663</idno><imprint><date when="2011">2011</date></imprint></series></biblStruct></sourceDesc><seriesStmt><title level="j" type="main">Biosensors & bioelectronics</title><title level="j" type="abbreviated">Biosens. bioelectron.</title><idno type="ISSN">0956-5663</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Antibody</term><term>Avian influenza</term><term>Avian influenzavirus</term><term>Crystals</term><term>Detection</term><term>H5N1</term><term>Immunosensor</term><term>Influenzavirus A(H5N1)</term><term>Magnetic</term><term>Quartz microbalance</term></keywords><keywords scheme="Pascal" xml:lang="fr"><term>Microbalance quartz</term><term>Immunodétecteur</term><term>Détection</term><term>Grippe aviaire</term><term>Magnétique</term><term>Influenzavirus aviaire</term><term>Cristal</term><term>Anticorps</term><term>Souche H5N1</term><term>Influenzavirus A(H5N1)</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">As a potential pandemic threat to human health, there has been an urgent need for rapid detection of the highly pathogenic avian influenza (AI) H5N1 virus. In this study, magnetic nanobeads amplification based quartz crystal microbalance (QCM) immunosensor was developed as a new method and application for AI H5N1 virus detection. Polyclonal antibodies against AI H5N1 virus surface antigen HA (Hemagglutinin) were immobilized on the gold surface of the QCM crystal through self-assembled monolayer (SAM) of 16-mercaptohexadecanoic acid (MHDA). Target H5N1 viruses were then captured by the immobilized antibodies, resulting in a change in the frequency. Magnetic nanobeads (diameter, 30 nm) coated with anti-H5 antibodies were used for further amplification of the binding reaction between antibody and antigen (virus). Both bindings of target H5N1 viruses and magnetic nanobeads onto the crystal surface were further confirmed by environmental scanning electron microscopy (ESEM). The QCM immunosensor could detect the H5N1 virus at a titer higher than 0.0128 HA unit within 2 h. The nanobeads amplification resulted in much better detection signal for target virus with lower titers. The response of the antibody-antigen (virus) interaction was shown to be virus titer-dependent, and a linear correlation between the logarithmic number of H5N1 virus titers and frequency shift was found from 0.128 to 12.8 HA unit. No significant interference was observed from non-target subtypes such as AI subtypes H3N2, H2N2, and H4N8. The immunosensor was evaluated using chicken tracheal swab samples. This research demonstrated that the magnetic nanobeads amplification based QCM immunosensor has a great potential to be an alternative method for rapid, sensitive, and specific detection of AI virus H5N1 in agricultural, food, environmental and clinical samples.</div></front></TEI><affiliations><list><country><li>République populaire de Chine</li><li>États-Unis</li></country><region><li>Arkansas</li><li>Pennsylvanie</li><li>Texas</li><li>Zhejiang</li></region><settlement><li>Hangzhou</li></settlement><orgName><li>Université de Zhejiang</li></orgName></list><tree><country name="République populaire de Chine"><region name="Zhejiang"><name sortKey="Dujuan Li" sort="Dujuan Li" uniqKey="Dujuan Li" last="Dujuan Li">DUJUAN LI</name></region><name sortKey="Jianping Wang" sort="Jianping Wang" uniqKey="Jianping Wang" last="Jianping Wang">JIANPING WANG</name></country><country name="États-Unis"><region name="Arkansas"><name sortKey="Dujuan Li" sort="Dujuan Li" uniqKey="Dujuan Li" last="Dujuan Li">DUJUAN LI</name></region><name sortKey="Abi Ghanem, Daad" sort="Abi Ghanem, Daad" uniqKey="Abi Ghanem D" first="Daad" last="Abi-Ghanem">Daad Abi-Ghanem</name><name sortKey="Berghman, Luc" sort="Berghman, Luc" uniqKey="Berghman L" first="Luc" last="Berghman">Luc Berghman</name><name sortKey="Hargis, Billy" sort="Hargis, Billy" uniqKey="Hargis B" first="Billy" last="Hargis">Billy Hargis</name><name sortKey="Huaguang Lu" sort="Huaguang Lu" uniqKey="Huaguang Lu" last="Huaguang Lu">HUAGUANG LU</name><name sortKey="Ronghui Wang" sort="Ronghui Wang" uniqKey="Ronghui Wang" last="Ronghui Wang">RONGHUI WANG</name><name sortKey="Yanbin Li" sort="Yanbin Li" uniqKey="Yanbin Li" last="Yanbin Li">YANBIN LI</name><name sortKey="Yanbin Li" sort="Yanbin Li" uniqKey="Yanbin Li" last="Yanbin Li">YANBIN LI</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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